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Westernbright ECL has high sensitivity for detection of low-abundance proteins WesternBright ECL is several times more sensitive than Amersham ECL. Both blots were simultaneously exposed to the same sheet of film for 15 seconds. Duplicate slot blots containing serial dilutions of transferrin protein were detected using WesternBright ECL or Amersham™ ECL™ (GE Healthcare). WesternBright ECL substrate produces a strong, long-lasting signal after reaction with HRP. WesternBright ECL is specially formulated to produce a strong signal for sensitive film imaging. WesternBright ECL is optimized for chemiluminescent Western blots imaged using X-ray film. Additionally, the WesternBright ECL signal is long lasting, allowing multiple exposures without substantial signal decay. WesternBright ECL is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to save precious antibodies and samples. M, Magic Mark XP western protein standard, 2 µl/lane (Invitrogen, Carlsbad, CA).WesternBright ® ECL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using X-ray film. All images were unaltered for brightness and contrast. (C and D) Both immunoblots in (A and B) were set-aside for 1 hr at which time they were re-exposed for 10 min. An immunoreactive band corresponding to actin (42 kDa) was detected in both instances. Both blots were exposed for 1 min in a FujiFilm LAS-4000 mini luminescent image analyzer (GE Life Sciences, Piscataway, NJ). (A and B) Immunoblots incubated in commercial ECL reagent (A) and self-prepared ECL reagent (B) as described above. The membrane was cut into two halves, and each half was incubated in ECL reagent. After washing, the membrane was incubated in bovine anti-goat IgG-HRP, followed by extensive washing as described above. The nitrocellulose membrane was incubated in anti-actin IgG for ∼3 hr at R.T. with gentle agitation on an orbital shaker, followed by a 10-min washing step in which the wash buffer was changed five times to remove all traces of non-fat milk. Thereafter, the nitrocellulose membrane was blocked in 5% non-fat milk (w/v) dissolved in wash buffer for 1 hr at R.T. Increasing concentrations of protein ranging from 1 to 50 µg total protein (protein estimation was performed by using the Pierce BCA protein assay kit Thermo Scientific, Rockford, IL) were resolved by SDS-PAGE under reducing conditions and transferred onto a nitrocellulose membrane (BIO-RAD, Hercules, CA) for 4 hr. We also include some helpful tips on how to avoid high background during immunoblotting.Ī comparison between commercial and self-prepared ECL reagents. 1) and present this protocol which is based on an earlier publication but with minor modifications, 6 hoping that it is useful for investigators in the field. In this technical report, we summarize our findings ( Fig. After scanning the literature carefully, testing chemicals from different vendors and trying different experimental conditions, we show that there exists an excellent alternative to commercially-available enhanced chemiluminescence kits. Consequently, these pricey kits can put enormous strain on any investigator managing a multi-person lab but with a limited supply budget. The most popular method used to visualize a protein at the nanogram level involves enhanced chemiluminescence, and several easy-to-use detection kits are commercially available to accomplish this goal, except that these kits are expensive and reagents have a relatively short shelf-life. Thus, it is a technique used by a vast number of laboratories, including ours, 2 – 5 to generate meaningful and interpretable results with relative ease. 1 For example, immunoblotting can be used to effectively assess changes in the protein level of any target gene following a specific cellular treatment, following overexpression or knockout/knockdown of a gene or following co-immunoprecipitation to study different scientific disciplines including spermatogenesis. Immunoblotting (i.e., western blotting) is a powerful technique used to detect a specific protein within a given sample or set of samples. Method 6: Razer Cortex from the Registry. Sometimes, the installer may allow you to repair or uninstall the program easily. Run the installer either in the original disk or the download file to reinstall the program again. In such cases, reinstalling Razer Cortex may do the trick. When the files required to uninstall Razer Cortex are corrupted or missing, it will not be able to remove the program. Method 5: Reinstall Razer Cortex to Uninstall. exe of any software with an antivirus before installing it. If you cannot uninstall Razer Cortex like other programs, then it’s worth checking. They can easily bypass the detection of the antivirus programs installed on your system. They usually get installed on your system by bundling with freeware software like fake apps, games or PDF convertors. There are lots of malware programs available online and most of them are probably unwanted malicious programs are also very difficult to remove. Such malware get into the computer with the assistance of the Trojans and spyware. These days, laptop malware appear like common computer app but they are much more strenuous to uninstall from the computer. Method 4: Uninstall Razer Cortex with Antivirus. You should backup your personal files and data before performing a System Restore. If you have executed a system restore point initially to installing a app, then you can utilize System Restore to restore your system and completely remove the unwanted programs like Razer Cortex. It is a utility of the Windows Operating System which comes with Windows operating systems and computer users to reform the system to a former state like a way back machine and remove programs interfering with the operation of the computer. Windows PC comes with lots of advanced features and System Restore is one of them. Method 3: Remove Razer Cortex via System Restore. You can find these files in the installation folder of Razer Cortex. Most of the Pc programs have an executable file named uninst000.exe or uninstall.exe or something along these lines. Method 2: Remove Razer Cortex with its uninstaller.exe. So when you want to uninstall Razer Cortex, the first thing is to uninstall it via Programs and Features. When you want to uninstall the program, you can go to the Programs and Features to get rid of it. When a software is installed on your Pc, that program is linked to the catalogue in Programs and Features. How to Uninstall Razer Cortex Completley? Method 1: Uninstall Razer Cortex via Programs and Features. So, it’s really important to completely uninstall Razer Cortex and remove all of its files and folders. An incomplete uninstallation of Razer Cortex may also cause many complications. Razer Cortex cannot be removed due to different problems. Uninstallation process may trigger error ‘ another process that is using the file stops Razer Cortex being uninstalled‘.Not all of the files were successfully uninstalled.You may also get error ‘ file required for the uninstallation to complete could not be run‘.This is the most common error.One of the most pumping error is ‘ You do not have sufficient access to remove Razer Cortex‘.Razer Cortex is not visible in the Programs and Features of the system.Various Errors While Uninstalling Razer Cortex Razer Cortex file location in Windows 7,8 and 10.Razer Cortex showing error while uninstalling?.Method 7: Uninstall Razer Cortex with Third-party Cleaners.Method 6: Razer Cortex from the Registry.Method 5: Reinstall Razer Cortex to Uninstall.Method 4: Uninstall Razer Cortex with Antivirus.Method 3: Remove Razer Cortex via System Restore.Method 2: Remove Razer Cortex with its uninstaller.exe.Method 1: Uninstall Razer Cortex via Programs and Features. 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